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Product Info Summary
| SKU: | EKC2466 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
Product info
Product Name
mTOR (Phospho-Ser2448) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2466
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The mTOR (Phospho-Ser2448) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor mTOR (Phospho-Ser2448) protein expression profile in cells. The kit can be used for measuring the relative amounts of mTOR (Phospho-Ser2448) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on mTOR (Phospho-Ser2448).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
mTOR (Phospho-Ser2448) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2466)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2466 is reactive to MTOR in Human, Mouse, Rat samples
Application Guarantee
EKC2466 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 30 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 60 ml (15x) | Clear |
| 100x Anti-mTOR Phospho-Ser2448 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-mTOR Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30'
Protein Target Info & Infographic
Gene/Protein Information For MTOR (Source: Uniprot.Org, NCBI)
Gene Name
MTOR
Full Name
Weight
288.9 kDa
Alternative Names
Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1;
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MTOR, check out the MTOR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MTOR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
Specific Publications For mTOR (Phospho-Ser2448) Colorimetric Cell-Based ELISA Kit (EKC2466)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for mTOR (Phospho-Ser2448) Colorimetric Cell-Based ELISA Kit
Question
Which one is better for comparing phosphorylation, EKC2319 or EKC2466?
Verified customer
Asked: 2019-07-19
Answer
The MTOR (Phospho-Ser2448) Colorimetric Cell-Based ELISA Kit (EKC2466) and MTOR (Phospho-Thr2446) Colorimetric Cell-Based ELISA Kit (EKC2319) are designed to detected mTOR phosphorylation, therefore, both should work well in detecting phosphorylated mTOR. One thing to consider is which phospho site is being studied.
Boster Scientific Support
Answered: 2019-07-20
