NPFF1 Receptor (NPFFR1) (NM_022146) Human Over-expression Lysate

Lysate of Neuropeptide FF R1/NPFFR1 over-expression cell lines, WB control

Transient overexpression lysate of neuropeptide FF receptor 1 (NPFFR1)

Product Info Summary

SKU: LS064106
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of neuropeptide FF receptor 1 (NPFFR1)

Customers Who Bought This Also Bought

Product Name

NPFF1 Receptor (NPFFR1) (NM_022146) Human Over-expression Lysate

See all Neuropeptide FF R1/NPFFR1 products

SKU/Catalog Number

LS064106

Size

100 µg

Description

Transient overexpression lysate of neuropeptide FF receptor 1 (NPFFR1)

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

NPFF1 Receptor (NPFFR1) (NM_022146) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS064106)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for NPFFR1 (Source: Uniprot.org, NCBI)

Gene Name

NPFFR1

Full Name

Neuropeptide FF receptor 1

Weight

47.819kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

FLJ10751; G protein-coupled receptor 147; GPR147; neuropeptide FF 1; Neuropeptide FF R1; neuropeptide FF receptor 1; Neuropeptide FFR1; NPFF1; NPFF1G-protein coupled receptor 147; NPFF1R1; NPFFR1; OT7T022; RFamide-related peptide receptor OT7T022 NPFFR1 GPR147, NPFF1, NPFF1R1, OT7T022 neuropeptide FF receptor 1 neuropeptide FF receptor 1|G-protein coupled receptor 147|RFamide-related peptide receptor OT7T022|neuropeptide FF 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on NPFFR1, check out the NPFFR1 Infographic

NPFFR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NPFFR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Loading publications

No publications found

Do you have publications using this product? Share with us and receive a reward. Contact us for more information.

Publications not available. Please try again later.

Product has been cited in publications

No results found matching your query

  • Published: --- Journal: --- Application: --- Impact Factor: --- Species: --- PMID: None

Have you used NPFF1 Receptor (NPFFR1) (NM_022146) Human Over-expression Lysate?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For NPFF1 Receptor (NPFFR1) (NM_022146) Human Over-expression Lysate

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for NPFF1 Receptor (NPFFR1) (NM_022146) Human Over-expression Lysate

$960
Backordered.

Lead time for this item is typically 2 weeks

Get A Quote
In stock
Order Product
LS064106
$960.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.