p15 INK4b (CDKN2B) (NM_004936) Human Over-expression Lysate

Lysate of p15INK4b/CDKN2B over-expression cell lines, WB control

Transient overexpression lysate of cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1

Product Info Summary

SKU: LS001030
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1

Product Name

p15 INK4b (CDKN2B) (NM_004936) Human Over-expression Lysate

See all p15INK4b/CDKN2B products

SKU/Catalog Number

LS001030

Size

100 µg

Description

Transient overexpression lysate of cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1

Storage & Handling

Cite This Product

p15 INK4b (CDKN2B) (NM_004936) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001030)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for CDKN2B (Source: Uniprot.org, NCBI)

Gene Name

CDKN2B

Full Name

Cyclin-dependent kinase 4 inhibitor B

Weight

14.722kDa

Superfamily

CDKN2 cyclin-dependent kinase inhibitor family

Alternative Names

CDK4I; CDKN2B; cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); cyclin-dependent kinases 4 and 6 binding protein; INK4BCDK4B inhibitor; MTS2; MTS-2; MTS2CDK inhibitory protein; Multiple tumor suppressor 2; p14_CDK inhibitor; p14_INK4B; p14-INK4b; p15 CDK inhibitor; p15_INK4B; P15cyclin-dependent kinase 4 inhibitor B; p15INK4b; p15-INK4b; TP15 CDKN2B CDK4I, INK4B, MTS2, P15, TP15, p15INK4b cyclin dependent kinase inhibitor 2B cyclin-dependent kinase 4 inhibitor B|CDK inhibitory protein|CDK4B inhibitor|MTS-2|cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)|cyclin-dependent kinases 4 and 6 binding protein|multiple tumor suppressor 2|p14-INK4b|p14_CDK inhibitor|p14_INK4B|p15 CDK inhibitor|p15-INK4b|p15_INK4B

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on CDKN2B, check out the CDKN2B Infographic

CDKN2B infographic

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In this infographic, you will see the following information for CDKN2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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