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Product Info Summary
| SKU: | LS001432 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of mitogen-activated protein kinase 14 (MAPK14), transcript variant 1 |
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Product info
Product Name
p38 (MAPK14) (NM_001315) Human Over-expression Lysate
SKU/Catalog Number
LS001432
Size
100 µg
Description
Transient overexpression lysate of mitogen-activated protein kinase 14 (MAPK14), transcript variant 1
Storage & Handling
Cite This Product
p38 (MAPK14) (NM_001315) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001432)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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p38 (MAPK14) Human Over-expression Lysates NM_001315.
Western blot validation of overexpression lysate (LS001432) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MAPK14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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p38 (MAPK14) Human Over-expression Lysates NM_139012.
Western blot validation of overexpression lysate (LS001432) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MAPK14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
p38 (MAPK14) Human Over-expression Lysates NM_139013.
Western blot validation of overexpression lysate (LS001432) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MAPK14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for MAPK14 (Source: Uniprot.org, NCBI)
Gene Name
MAPK14
Full Name
Mitogen-activated protein kinase 14
Weight
41.293kDa
Superfamily
protein kinase superfamily
Alternative Names
MAPK14; p38 alpha; SAPK2a MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MAPK14, check out the MAPK14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For p38 (MAPK14) (NM_001315) Human Over-expression Lysate (LS001432)
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