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- Table of Contents
MAPK14 antibody (M00176-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody
MAPK14 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
MAPK14 antibody was positively detected by WB in:
human Jurkat whole cell, human SW620 whole cell, human CACO-2 whole cell, human Hela whole cell, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MAPK14 antibody was positively detected by IHC in:
human brain tissue, Human thyroid cancer tissue, Human prostate cancer tissue, Human astrocytoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody
View all MAPK14 Antibodies
View all MAPK14 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EGC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p38 MAPK
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
41, 38 kDa
Calculated Molecular Weight
41.3 kDa
Cite This Product
Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00176-1)
Protocols
Western Blot protocol for MAPK14 antibody
Immunohistochemistry (IHC) protocol for MAPK14 antibody
Publications
Application:
Species:
Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells
Published: 2025-11-29
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 41325790
Farnesyl Pyrophosphate Synthase Promotes Restenosis After Vascular Injury by Activating Small G Proteins
Published: 2025-10-27
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB
Impact Factor: 3.90
Species: Rat
PMID: 41161388
Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study
Published: 2024-06-06
Journal: Heliyon
Application: WB
Impact Factor: 3.40
Species: Mouse
PMID: 38952369
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway
Published: 2024-04-16
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38636574
Total Panax notoginseng saponin inhibits balloon injury-induced neointimal hyperplasia in rat carotid artery models by suppressing pERK/p38 MAPK pathways
Published: 2019-12-20
Journal: BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
Application: WB,IHC
Impact Factor: 1.85
Species: Rat
PMID: 31859914
Deoxynivalenol induces toxicity and apoptosis in piglet hippocampal nerve cells via the MAPK signaling pathway
Published: 2018-10-02
Journal: TOXICON
Application: WB
Impact Factor: 2.35
Species: Pig
PMID: 30290166
Review
Have you used Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody?
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1 Review For Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody
Anti-MAPK14 Antibody (M00176-1) yielded distinct target bands at the correct molecular size with low background, alongside faint non-specific bands. Western blot results demonstrated elevated MAPK14 levels in Aβ-challenged PC-12 AD model cells, while drug
| SKU | M00176-1 |
|---|---|
| Application | Western Blot |
| Sample | PC-12 (rat adrenal pheochromocytoma cells, highly differentiated) |
| Sample Processing Description | ① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | p38 MAPK MAPK14 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2500, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | ① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed clear target bands. After modeling, the total expression level of MAPK14 protein was upregulated, and the expression level decreased after drug treatment, which was in line with expectations. |
Yue Zhang, Chengdu University of Traditional Chinese Medicine
Verified customer
Submitted 2026-08-26
Product Promise
We promise Anti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00176-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026