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Product Info Summary
|Description:||Transient overexpression lysate of aldehyde dehydrogenase 18 family, member A1 (ALDH18A1), nuclear gene encoding mitochondrial protein, transcript variant 1|
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P5CS (ALDH18A1) (NM_002860) Human Over-expression Lysate
Transient overexpression lysate of aldehyde dehydrogenase 18 family, member A1 (ALDH18A1), nuclear gene encoding mitochondrial protein, transcript variant 1
Storage & Handling
Cite This Product
P5CS (ALDH18A1) (NM_002860) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005832)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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P5CS (ALDH18A1) Human Over-expression Lysates NM_002860.
Western blot validation of overexpression lysate (LS005832) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ALDH18A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ALDH18A1 (Source: Uniprot.org, NCBI)
aldehyde dehydrogenase 18 family, member A1; Aldehyde dehydrogenase family 18 member A1; delta-1-pyrroline-5-carboxylate synthase; EC 126.96.36.199; EC 188.8.131.52; GSASdelta1-pyrroline-5-carboxlate synthetase; MGC117316; P5CSdelta-1-pyrroline-5-carboxylate synthetase; PYCSEC 184.108.40.206; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase) ALDH18A1 ADCL3, ARCL3A, GSAS, P5CS, PYCS, SPG9, SPG9A, SPG9B aldehyde dehydrogenase 18 family member A1 delta-1-pyrroline-5-carboxylate synthase|Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux)|aldehyde dehydrogenase family 18 member A1|delta-1-pyrroline-5-carboxylate synthetase|delta1-pyrroline-5-carboxlate synthetase|pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)|spastic paraplegia 9 (autosomal dominant)*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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