PI 3 Kinase p85 alpha (PIK3R1) (NM_181504) Human Over-expression Lysate

Product Name

PI 3 Kinase p85 alpha (PIK3R1) (NM_181504) Human Over-expression Lysate

See all PI 3-Kinase p85 alpha products

SKU/Catalog Number

LS005295

Size

100 µg

Description

Transient overexpression lysate of phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 2

Storage & Handling

Cite This Product

PI 3 Kinase p85 alpha (PIK3R1) (NM_181504) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005295)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for PIK3R1 (Source: Uniprot.org, NCBI)

Alternative Names

GRB1; GRB1PI3-kinase subunit p85-alpha; p85; p85-ALPHA; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory, 1; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI 3Kinase p85 alpha; PI 3-Kinase p85 alpha; PI3K regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PIK3R1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory s PIK3R1 AGM7, GRB1, IMD36, p85, p85-ALPHA phosphoinositide-3-kinase regulatory subunit 1 phosphatidylinositol 3-kinase regulatory subunit alpha|PI3-kinase subunit p85-alpha|PI3K regulatory subunit alpha|phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase regulatory subunit alpha|phosphoinositide-3-kinase, regulatory subunit 1 (alpha)|ptdIns-3-kinase regulatory subunit alpha

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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