Product Info Summary
SKU: | LS005293 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of phosphoinositide-3-kinase, catalytic, delta polypeptide (PIK3CD) |
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Product info
Product Name
PIK3CD (NM_005026) Human Over-expression Lysate
See all PI 3-Kinase p110 delta products
SKU/Catalog Number
LS005293
Size
100 µg
Description
Transient overexpression lysate of phosphoinositide-3-kinase, catalytic, delta polypeptide (PIK3CD)
Storage & Handling
Cite This Product
PIK3CD (NM_005026) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005293)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PIK3CD Human Over-expression Lysates NM_005026.
Western blot validation of overexpression lysate (LS005293) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PIK3CD (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PIK3CD (Source: Uniprot.org, NCBI)
Gene Name
PIK3CD
Full Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
Weight
119.479kDa
Superfamily
PI3/PI4-kinase family
Alternative Names
EC 2.7.1; EC 2.7.1.153; p110D; P110DELTA; Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphoinositide-3-kinase C; phosphoinositide-3-kinase, catalytic, delta polypeptide; PI 3Kinase p110 delta; PI 3-Kinase p110 delta; PI3K; PI3K-delta; PI3-kinase p110 subunit delta; PI3-kinase subunit delta; PIK3CD; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta PIK3CD APDS, IMD14, P110DELTA, PI3K, p110D phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform|PI3-kinase p110 subunit delta|PI3Kdelta|phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta|phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 20|phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8|phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8/20|phosphoinositide-3-kinase C|phosphoinositide-3-kinase, catalytic, delta polypeptide variant p37delta|ptdIns-3-kinase subunit p110-delta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PIK3CD, check out the PIK3CD Infographic
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In this infographic, you will see the following information for PIK3CD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PIK3CD (NM_005026) Human Over-expression Lysate (LS005293)
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