Porcine IL-1 beta ELISA Kit PicoKine®

Product Name

Porcine IL-1 beta ELISA Kit PicoKine®

SKU/Catalog Number

EK2348

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Porcine IL-1 beta ELISA Kit PicoKine® (96 Tests). Quantitate Porcine IL-1 beta in cell culture supernatants, serum, plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Porcine IL-1 beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2348)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: Ala115-Pro267

Capture/Detection Antibodies

polyclonal antibody from goat|polyclonal antibody from goat

Sensitivity

<10 pg/mL

Assay Range

39.1-2,500 pg/mL

Standard Dilution Instructions

See datasheet of EK2348 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2348 is reactive to IL1B in Porcine samples

Validated Sample Types

cell culture supernatants, serum, plasma(heparin)

Application Guarantee

EK2348 is guaranteed for ELISA in Porcine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL1B

Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Kit Components

Catalog Number	Description	Quantity
EK2348-CAP	Anti-Porcine IL1B Pre-coated 96-well strip microplate	1
EK2348-ST	Porcine IL1B Standard	2 vials, 10 ng/tube
EK2348-DA	Porcine IL1B Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	39	78	156	312.5	625	1250	2500
O.D.	0.015	0.132	0.221	0.437	0.757	1.307	2.007	2.448

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Porcine IL-1 beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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