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Product Info Summary
| SKU: | LS005721 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of proteasome (prosome, macropain) activator subunit 2 (PA28 beta) (PSME2) |
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Product info
Product Name
PSME2 (NM_002818) Human Over-expression Lysate
See all PA28 Activator beta Subunit/PSME2 products
SKU/Catalog Number
LS005721
Size
100 µg
Description
Transient overexpression lysate of proteasome (prosome, macropain) activator subunit 2 (PA28 beta) (PSME2)
Storage & Handling
Cite This Product
PSME2 (NM_002818) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005721)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PSME2 Human Over-expression Lysates NM_002818.
Western blot validation of overexpression lysate (LS005721) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PSME2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PSME2 (Source: Uniprot.org, NCBI)
Gene Name
PSME2
Full Name
Proteasome activator complex subunit 2
Weight
27.402kDa
Superfamily
PA28 family
Alternative Names
11S regulator complex beta subunit; 11S regulator complex subunit beta; Activator of multicatalytic protease subunit 2; MCP Activator; MCP activator, 31-kD subunit; PA28 Activator beta Subunit; PA28b; PA28betacell migration-inducing protein 22; proteasome (prosome, macropain) activator subunit 2 (PA28 beta); Proteasome activator 28 subunit beta; proteasome activator 28-beta; proteasome activator complex subunit 2; proteasome activator hPA28 subunit beta; PSME2; REGbeta; REG-beta PSME2 PA28B, PA28beta, REGbeta proteasome activator subunit 2 proteasome activator complex subunit 2|11S regulator complex beta subunit|11S regulator complex subunit beta|MCP activator, 31-kD subunit|REG-beta|activator of multicatalytic protease subunit 2|cell migration-inducing protein 22|proteasome (prosome, macropain) activator subunit 2 (PA28 beta)|proteasome activator 28 subunit beta|proteasome activator 28-beta|proteasome activator hPA28 subunit beta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PSME2, check out the PSME2 Infographic
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In this infographic, you will see the following information for PSME2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PSME2 (NM_002818) Human Over-expression Lysate (LS005721)
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