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Product Info Summary
|Description:||Transient overexpression lysate of Rab geranylgeranyltransferase, beta subunit (RABGGTB)|
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RABGGTB (NM_004582) Human Over-expression Lysate
Transient overexpression lysate of Rab geranylgeranyltransferase, beta subunit (RABGGTB)
Storage & Handling
Cite This Product
RABGGTB (NM_004582) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005876)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RABGGTB Human Over-expression Lysates NM_004582.
Western blot validation of overexpression lysate (LS005876) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RABGGTB (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RABGGTB (Source: Uniprot.org, NCBI)
Geranylgeranyl transferase type-2 subunit beta
protein prenyltransferase subunit beta family
EC 184.108.40.206; Geranylgeranyl transferase type II subunit beta; geranylgeranyl transferase type-2 subunit beta; GGTase-II-beta; GGTB; Rab geranylgeranyltransferase subunit beta; Rab geranyl-geranyltransferase subunit beta; Rab geranylgeranyltransferase, beta subunit; Rab GG transferase beta; Rab GGTase beta; Type II protein geranyl-geranyltransferase subunit beta RABGGTB GGTB Rab geranylgeranyltransferase subunit beta geranylgeranyl transferase type-2 subunit beta|GGTase-II-beta|Rab geranylgeranyltransferase beta subunit|rab GG transferase beta|rab GGTase beta|type II protein geranyl-geranyltransferase subunit beta*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS005876
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