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Product Info Summary
|Description:||Transient overexpression lysate of RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2|
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RAP1A (NM_002884) Human Over-expression Lysate
Transient overexpression lysate of RAP1A, member of RAS oncogene family (RAP1A), transcript variant 2
Storage & Handling
Cite This Product
RAP1A (NM_002884) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005906)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RAP1A Human Over-expression Lysates NM_002884.
Western blot validation of overexpression lysate (LS005906) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RAP1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RAP1A (Source: Uniprot.org, NCBI)
Ras-related protein Rap-1A
small GTPase superfamily
C21KG; G-22K; GTP-binding protein smg p21A; KREV-1; KREV1Ras-related protein Krev-1; RAP1; Rap1A; RAP1A, member of RAS oncogene family; RAS-related protein RAP1A; ras-related protein Rap-1A; SMGP21 RAP1A C21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21 RAP1A, member of RAS oncogene family ras-related protein Rap-1A|GTP-binding protein smg p21A|Ras-related protein Krev-1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on RAP1A, check out the RAP1A Infographic
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No publications found for LS005906
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