Product Info Summary
SKU: | LS065059 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (RAPH1), transcript variant 3 |
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Product info
Product Name
RAPH1 (NM_203365) Human Over-expression Lysate
SKU/Catalog Number
LS065059
Size
100 µg
Description
Transient overexpression lysate of Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (RAPH1), transcript variant 3
Storage & Handling
The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Cite This Product
RAPH1 (NM_203365) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS065059)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
RAPH1 Human Over-expression Lysates NM_203365.
Western blot validation of overexpression lysate (LS065059) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RAPH1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RAPH1 (Source: Uniprot.org, NCBI)
Gene Name
RAPH1
Full Name
Ras-associated and pleckstrin homology domains-containing protein 1
Weight
135.256kDa
Superfamily
MRL family
Alternative Names
candidate 18; candidate 9; lamellipodin; PREL2; PREL-2; proline rich EVH1 ligand 2; Protein RMO1; Ras association (RalGDS/AF-6) and pleckstrin homology domains 1; ras-associated and pleckstrin homology domains-containing protein 1; RMO1 RAPH1 ALS2CR18, ALS2CR9, LPD, PREL-2, PREL2, RMO1, RalGDS/AF-6 Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 ras-associated and pleckstrin homology domains-containing protein 1|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 18|amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 9|lamellipodin|proline-rich EVH1 ligand 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RAPH1, check out the RAPH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For RAPH1 (NM_203365) Human Over-expression Lysate (LS065059)
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