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Product Info Summary
| SKU: | EK0471 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Rat NGF/NGF Beta ELISA Kit PicoKine®
SKU/Catalog Number
EK0471
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat NGF/NGF Beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0471)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S122-G241
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0471 for more details
Cross-reactivity
There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.
Reactive Species
EK0471 is reactive to NGF in Rat samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0471 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of beta-NGF
Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0471-CAP | Anti-Rat NGF Pre-coated 96-well strip microplate | 1 |
| EK0471-ST | Rat NGF Standard | 2 vials, 10 ng/tube |
| EK0471-DA | Rat NGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.079 | 0.133 | 0.190 | 0.296 | 0.521 | 0.876 | 1.414 | 2.025 |
Data Example Images
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Rat NGF/NGF beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect beta-NGF, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of beta-NGF in samples: 15615500 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat NGF/NGF Beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 191 | 480 | 29 | 201 | 491 |
| Standard deviation | 1.84 | 8.02 | 32.64 | 1.62 | 11.65 | 35.84 |
| CV (%) | 4.2% | 4.2% | 6.8% | 5.6% | 5.8% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of beta-NGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 32 | 33 | 35 | 33 | 1.08 | 3.2% |
| Sample 2 | 191 | 172 | 185 | 192 | 185 | 7.96 | 4.3% |
| Sample 3 | 480 | 451 | 440 | 436 | 451 | 17.21 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For NGF (Source: Uniprot.Org, NCBI)
Gene Name
NGF
Full Name
Beta-nerve growth factor
Weight
26.959kDa
Superfamily
NGF-beta family
Alternative Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NGF, check out the NGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat NGF/NGF Beta ELISA Kit PicoKine® (EK0471)
Hello CJ!
EK0471 has been cited in 36 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.
A novel tissue engineered nerve graft constructed with autologous vein and nerve microtissue repairs a long-segment sciatic nerve defect
Long-Term Running Exercise Delays Age-Related Changes in White Matter in Rats
The expressions of NGF and VEGF in the fracture tissues are closely associated with accelerated clavicle fracture healing in patients with traumatic brain injury
Triptolide improves neurobehavioral functions, inflammation, and oxidative stress in rats under deep hypothermic circulatory arrest
Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke
The Role of Neurotrophic Factors Conjugated to Iron Oxide Nanoparticles in Peripheral Nerve Regeneration: In Vitro Studies
Bionic microenvironment-inspired synergistic effect of anisotropic micro-nanocomposite topology and biology cues on peripheral nerve regeneration
Transitional changes in gastrointestinal transit and rectal sensitivity from active to recovery of inflammation in a rodent model of colitis
Enhancement of Schwann Cells Function Using Graphene-Oxide-Modified Nanofiber Scaffolds for Peripheral Nerve Regeneration
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
20 Customer Q&As for Rat NGF/NGF Beta ELISA Kit PicoKine®
Question
Can EK0471 detect dissociated NGF or total NGF?
Verified customer
Asked: 2022-08-08
Answer
The Rat NGF/NGF Beta ELISA Kit PicoKine® (EK0471) detects total NGF.
Boster Scientific Support
Answered: 2022-08-08
Question
Is this cross reactive with proNGF, or is it specific towards the mature form of NGF?
Verified customer
Asked: 2022-08-05
Answer
Thank you for your question. The immunogen sequence for the antibody pair in EK0471 is S122-G241 (Accession # P25427). It is specific towards the mature form of NGF.
Boster Scientific Support
Answered: 2022-08-05
Question
Is EK0471 a competitive or non-competitive antibody?
Verified customer
Asked: 2022-08-02
Answer
The Rat NGF/NGF Beta ELISA Kit PicoKine® (EK0471) is a non-competitive antibody.
Boster Scientific Support
Answered: 2022-08-02
Question
Is Rat NGF Standard in EK0471 monomer or dimer?
Verified customer
Asked: 2022-08-02
Answer
The standard in the Rat NGF/NGF Beta ELISA Kit PicoKine® (EK0471) is dimer.
Boster Scientific Support
Answered: 2022-08-02
Question
Has EK0471 been tested for cross-reactivity or interference between Ngf and the complex formed by Ngf and α2-macroglobulin?
Verified customer
Asked: 2022-05-04
Answer
For the Rat NGF/NGF Beta ELISA Kit PicoKine® (EK0471), our lab hasn't tested the cross-reactivity between Ngf and the complex formed by Ngf and α2-macroglobulin. We tested the following factors and no cross-reactivity or interference was observed: Recombinant rat CNTF, GDNF Recombinant human BDNF, CNTF, GDNF, NT-3, NT-4
Boster Scientific Support
Answered: 2022-05-06
Question
Does EK0471 focus more on the detection of a certain type of samples, animal serum samples or in vitro experimental samples?
Verified customer
Asked: 2022-05-04
Answer
The Rat NGF/NGF Beta ELISA Kit PicoKine® (EK0471) detects cell culture supernatants and serum.
Boster Scientific Support
Answered: 2022-05-06
Question
Does the capture antibody in EK0471 have neutralization functions?
Verified customer
Asked: 2022-03-15
Answer
The capture antibody in Rat NGF/NGF Beta ELISA Kit PicoKine® (EK0471) can be used for Neutralization application.
Boster Scientific Support
Answered: 2022-03-16
Question
Is the standard for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) natural material or recombinant?
Verified customer
Asked: 2020-12-09
Answer
For the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471), the standard is recombinant Rat beta-NGF standard and expression system is NS0.
Boster Scientific Support
Answered: 2020-12-10
Question
If the standard for EK0471 is recombinant, does it use a virus vector in the recombination and/or expression process? What kind of virus vector was used?
Verified customer
Asked: 2020-12-09
Answer
The Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) didn't use any virus vector in the recombination and/or expression process.
Boster Scientific Support
Answered: 2020-12-10
Question
Does the standard for EK0471 include additive agent such as antiseptic agent or preserving agent? What is composition of additive agent?
Verified customer
Asked: 2020-12-09
Answer
The standard for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) includes 0.02mg sodium azide. It is 0.38% in the lyophilized state.
Boster Scientific Support
Answered: 2020-12-10
Question
What is the material name and concentration of Stop Solution for EK0471?
Verified customer
Asked: 2020-12-09
Answer
The stop solution for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) is 2% Sulphuric acid.
Boster Scientific Support
Answered: 2020-12-10
Question
Does EK0471 include hazardous substance such as azide, thimerosal, 2-ME, not less than 1% surfactant like CAS# 9002-93-1 or CAS# 9016-45-9, etc? What kind of substance is included in which component and how much?
Verified customer
Asked: 2020-12-09
Answer
The the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) contains sodium azide less than 1%.
Boster Scientific Support
Answered: 2020-12-10
Question
How was the standard for EK0471 purified?
Verified customer
Asked: 2020-12-08
Answer
The standard for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) was affinity purified.
Boster Scientific Support
Answered: 2020-12-09
Question
Q: which procedure should I follow in order to thaw whole blood sample for beta-NGF ELISA after freezing?
R. Baker
Verified customer
Asked: 2019-11-08
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test beta-NGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-11-08
Question
Good day! I hope you are doing well. We received an inquiry about r.NGF/NGF (catalog# EK0471) from our customer today. Do you have a recommended dilution factor of serum sample? Or do you have any tested data for serum to share with the customer? Thank you very much for your help.
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
For EK0471, the recommended dilution factor of serum sample is 1:1.
Boster Scientific Support
Answered: 2019-09-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-10
Question
Q: can you recommend the dilution ratio of serum samples for detection of beta-NGF in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
W. Turner
Verified customer
Asked: 2018-12-02
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the beta-NGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-12-02
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-05-19
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-05-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-05-28
Question
Q: Can beta-NGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2014-07-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2014-07-01
