Rat RANK PicoKine™ Quick ELISA Kit

Rat RANK PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1281
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants and serum.
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Rat RANK PicoKine™ Quick ELISA Kit

See all RANK/TNFRSF11A products

SKU/Catalog Number

FEK1281

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat RANK PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat RANK PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1281)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-P213

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1281 is reactive to Tnfrsf11a in Rat samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

FEK1281 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity
FEK1281-CAP 96-well plate precoated with anti-Rat RANK/TNFRSF11A antibody 1
FEK1281-ST lyophilized recombinant Rat RANK/TNFRSF11A standard 10 ng/tube
FEK1281-DA Biotinylated anti-Rat RANK/TNFRSF11A detection antibody (50x) 130 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0150.1930.3520.5560.8851.4111.8302.023

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)20951414012265421518
Standard deviation13.5834.9588.1618.342.8197.15
CV (%)6.5%6.8%7.9%8.1%7.9%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of RANK/TNFRSF11A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120919418521720112.496.2%
Sample 2514489548539522234.4%
Sample 31401140614641575146170.054.7%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf11a (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y6Q6

Gene ID

8792

Gene Name

Tnfrsf11a

Full Name

Tumor necrosis factor receptor superfamily member 11A

Weight

66.034kDa

Alternative Names

CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator TNFRSF11A CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TRANCER TNF receptor superfamily member 11a tumor necrosis factor receptor superfamily member 11A|Paget disease of bone 2|loss of heterozygosity, 18, chromosomal region 1|osteoclast differentiation factor receptor|receptor activator of NF-KB|receptor activator of nuclear factor-kappa B|tumor necrosis factor receptor superfamily, member 11a, NFKB activator

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf11a, check out the Tnfrsf11a Infographic

Tnfrsf11a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf11a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for FEK1281

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Rat RANK PicoKine™ Quick ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Rat RANK PicoKine™ Quick ELISA Kit

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Rat RANK PicoKine™ Quick ELISA Kit

$499

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
FEK1281
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.