Rat Rantes PicoKine® Quick ELISA Kit

CCL5/RANTES ELISA kit, 90-minute one-step protocol

Rat Rantes PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Ccl5 in cell culture supernatants. serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Product Info Summary

SKU: FEK0496
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants. serum and plasma (heparin, EDTA, citrate)
Assay Time: 90 Minutes

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Product Name

Rat Rantes PicoKine® Quick ELISA Kit

See all CCL5/RANTES products

SKU/Catalog Number

FEK0496

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Rantes PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Ccl5 in cell culture supernatants. serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat Rantes PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0496)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-S91

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0496 is reactive to Ccl5 in Rat samples

Validated Sample Types

cell culture supernatants. serum and plasma (heparin, EDTA, citrate)

Application Guarantee

FEK0496 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL5/RANTES

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Kit Components

Catalog numberDescription QuantityVolume
FEK0496-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0496-STRat CCL5/RANTES Standard 2 10 ng/tube
FEK0496-CRat Ccl5 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0160.1640.2550.4500.8311.4452.0872.262

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3221236436199351
Standard deviation2.0216.3220.022.7414.1325.62
CV (%)6.3%7.7%5.5%7.6%7.1%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL5/RANTES in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 132303629321.655.2%
Sample 22121801962061999.734.9%
Sample33643974053743856.931.8%
*number of samples for each test n=16.

Gene/Protein Information For Ccl5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50231

Gene ID

81780

Gene Name

Ccl5

Full Name

C-C motif chemokine 5

Weight

10.17kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES Ccl5|Rantes, Scya5|C-C motif chemokine ligand 5|C-C motif chemokine 5|SIS-delta|T-cell-specific protein RANTES|chemokine (C-C motif) ligand 5|regulated upon activation normal T-cell expressed and secreted|small-inducible cytokine A5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl5, check out the Ccl5 Infographic

Ccl5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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