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Product Info Summary
|Description:||Transient overexpression lysate of Ras-like without CAAX 2 (RIT2)|
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RIT2 (NM_002930) Human Over-expression Lysate
Transient overexpression lysate of Ras-like without CAAX 2 (RIT2)
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Cite This Product
RIT2 (NM_002930) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006014)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RIT2 Human Over-expression Lysates NM_002930.
Western blot validation of overexpression lysate (LS006014) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RIT2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RIT2 (Source: Uniprot.org, NCBI)
GTP-binding protein Rit2
small GTPase superfamily
GTP-binding protein Rit2; GTP-binding protein Roc2; Ras-like protein expressed in neurons; Ras-like without CAAX 2; Ras-like without CAAX protein 2; RIBA; Ric (Drosophila)-like, expressed in neurons; Ric-like, expressed in neurons; RIN; Rit2; ROC2 RIT2 RIBA, RIN, ROC2 Ras like without CAAX 2 GTP-binding protein Rit2|GTP-binding protein Roc2|Ric-like, expressed in neurons|ras-like protein expressed in neurons|ras-like without CAAX protein 2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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