robld3 (LAMTOR2) (NM_014017) Human Over-expression Lysate

Lysate of ROBLD3 over-expression cell lines, WB control

Transient overexpression lysate of roadblock domain containing 3 (ROBLD3), transcript variant 1

Product Info Summary

SKU: LS028956
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of roadblock domain containing 3 (ROBLD3), transcript variant 1

Product Name

robld3 (LAMTOR2) (NM_014017) Human Over-expression Lysate

See all ROBLD3 products

SKU/Catalog Number

LS028956

Size

100 µg

Description

Transient overexpression lysate of roadblock domain containing 3 (ROBLD3), transcript variant 1

Storage & Handling

Cite This Product

robld3 (LAMTOR2) (NM_014017) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS028956)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for LAMTOR2 (Source: Uniprot.org, NCBI)

Gene Name

LAMTOR2

Full Name

Ragulator complex protein LAMTOR2

Weight

13.508kDa

Superfamily

GAMAD family

Alternative Names

ENDAP; Endosomal adaptor protein p14; HSPC003; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; late endosomal/lysosomal adaptor, MAPK and MTOR activator 2; Late endosomal/lysosomal Mp1-interacting protein; MAPBPIPRoadblock domain-containing protein 3; MAPKSP1 adaptor protein; MAPKSP1AP; mitogen-activated protein-binding protein-interacting protein (MAPBPIP); Mitogen-activated protein-binding protein-interacting protein; p14Ragulator2; roadblock domain containing 3; ROBLD3 LAMTOR2 ENDAP, HSPC003, MAPBPIP, MAPKSP1AP, ROBLD3, Ragulator2, p14 late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 ragulator complex protein LAMTOR2|MAPBP-interacting protein|MAPKSP1 adaptor protein|endosomal adaptor protein p14|late endosomal/lysosomal Mp1-interacting protein|late endosomal/lysosomal adaptor and MAPK and MTOR activator 2|mitogen-activated protein-binding protein-interacting protein (MAPBPIP)|roadblock domain containing 3|roadblock domain-containing protein 3

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on LAMTOR2, check out the LAMTOR2 Infographic

LAMTOR2 infographic

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In this infographic, you will see the following information for LAMTOR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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