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Product Info Summary
|Description:||Transient overexpression lysate of RAR-related orphan receptor B (RORB)|
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ROR beta (RORB) (NM_006914) Human Over-expression Lysate
Transient overexpression lysate of RAR-related orphan receptor B (RORB)
Storage & Handling
Cite This Product
ROR beta (RORB) (NM_006914) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006096)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ROR beta (RORB) Human Over-expression Lysates NM_006914.
Western blot validation of overexpression lysate (LS006096) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RORB (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RORB (Source: Uniprot.org, NCBI)
Nuclear receptor ROR-beta
nuclear hormone receptor family
NR1F2; NR1F2nuclear receptor ROR-beta; Nuclear receptor RZR-beta; Nuclear receptor subfamily 1 group F member 2; RAR-related orphan receptor B; RAR-related orphan receptor beta; retinoic acid-binding receptor beta; retinoid-related orphan receptor beta; Retinoid-related orphan receptor-beta; ROR beta; RORB; ROR-BETA; RZRB; RZRBbA133M9.1; RZR-BETA RORB EIG15, NR1F2, ROR-BETA, RZR-BETA, RZRB, bA133M9.1 RAR related orphan receptor B nuclear receptor ROR-beta|RAR-related orphan receptor beta|nuclear receptor RZR-beta|nuclear receptor subfamily 1 group F member 2|retinoic acid-binding receptor beta|retinoid-related orphan receptor beta*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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