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Product Info Summary
|Description:||Transient overexpression lysate of ribosomal protein L17 (RPL17), transcript variant 2|
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RPL17 (NM_001035006) Human Over-expression Lysate
Transient overexpression lysate of ribosomal protein L17 (RPL17), transcript variant 2
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Cite This Product
RPL17 (NM_001035006) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006139)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RPL17 Human Over-expression Lysates NM_001035006.
Western blot validation of overexpression lysate (LS006139) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPL17 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RPL17 (Source: Uniprot.org, NCBI)
60S ribosomal protein L17
universal ribosomal protein uL22 family
FLJ18762,60S ribosomal protein L23; FLJ92089; gene encoding putative NFkB activating protein; MGC117162; PD-1; Ribosomal Protein L17; RPL17; RPL23; RPL23,60S ribosomal protein L17 RPL17 L17, PD-1, RPL23 ribosomal protein L17 60S ribosomal protein L17|60S ribosomal protein L23|gene encoding putative NFkB activating protein|large ribosomal subunit protein uL22*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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