RSK1 p90 (RPS6KA1) (NM_002953) Human Over-expression Lysate

Lysate of RSK1 over-expression cell lines, WB control

Transient overexpression lysate of ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1

Product Info Summary

SKU: LS006195
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1

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Product Name

RSK1 p90 (RPS6KA1) (NM_002953) Human Over-expression Lysate

See all RSK1 products

SKU/Catalog Number

LS006195

Size

100 µg

Description

Transient overexpression lysate of ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1

Storage & Handling

Cite This Product

RSK1 p90 (RPS6KA1) (NM_002953) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006195)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for RPS6KA1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q15418

Gene ID

6195

Gene Name

RPS6KA1

Full Name

Ribosomal protein S6 kinase alpha-1

Weight

82.723kDa

Superfamily

protein kinase superfamily

Alternative Names

90kD, polypeptide 1); EC 2.7.11; EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1; HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPL1A; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK1; RSK-1; RSK1p90-RSK 1; S6K-alpha 1; S6K-alpha-1 RPS6KA1 HU-1, MAPKAPK1, MAPKAPK1A, RSK, RSK1, p90Rsk ribosomal protein S6 kinase A1 ribosomal protein S6 kinase alpha-1|90 kDa ribosomal protein S6 kinase 1|MAP kinase-activated protein kinase 1a|MAPK-activated protein kinase 1a|MAPKAP kinase 1a|MAPKAPK-1a|RSK-1|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|p90RSK1|p90S6K|ribosomal S6 kinase 1|ribosomal protein S6 kinase, 90kDa, polypeptide 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on RPS6KA1, check out the RPS6KA1 Infographic

RPS6KA1 infographic

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In this infographic, you will see the following information for RPS6KA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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