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Product Info Summary
|Description:||Transient overexpression lysate of S100 calcium binding protein A12 (S100A12)|
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S100A12 (NM_005621) Human Over-expression Lysate
Transient overexpression lysate of S100 calcium binding protein A12 (S100A12)
Storage & Handling
Cite This Product
S100A12 (NM_005621) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006283)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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S100A12 Human Over-expression Lysates NM_005621.
Western blot validation of overexpression lysate (LS006283) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with S100A12 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for S100A12 (Source: Uniprot.org, NCBI)
CAAF1; CAAF1Neutrophil S100 protein; CAAFI; CAGC; CAGCS100 calcium binding protein A12 (calgranulin C); CAGCS100; Calcium-binding protein in amniotic fluid 1; Calgranulin C; calgranulin-C; CGRPEN-RAGE; ENRAGE; EN-RAGE; Extracellular newly identified RAGE-binding protein; MRP6; p6calgranulin C; protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12 S100A12 CAAF1, CAGC, CGRP, ENRAGE, MRP-6, MRP6, p6 S100 calcium binding protein A12 protein S100-A12|EN-RAGE|calcitermin|calcium-binding protein in amniotic fluid 1|calgranulin C|extracellular newly identified RAGE-binding protein|migration inhibitory factor-related protein 6|neutrophil S100 protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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