SEMA6D (NM_153618) Human Over-expression Lysate

Lysate of Semaphorin 6D over-expression cell lines, WB control

Transient overexpression lysate of sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 4

Product Info Summary

SKU: LS080031
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 4

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Product Name

SEMA6D (NM_153618) Human Over-expression Lysate

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SKU/Catalog Number

LS080031

Size

100 µg

Description

Transient overexpression lysate of sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 4

Storage & Handling

Cite This Product

SEMA6D (NM_153618) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080031)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SEMA6D (Source: Uniprot.org, NCBI)

Gene Name

SEMA6D

Full Name

Semaphorin-6D

Weight

119.872kDa

Superfamily

semaphorin family

Alternative Names

KIAA1479FLJ11598; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D; Sema6D; Semaphorin 6D; semaphorin-6D SEMA6D semaphorin 6D semaphorin-6D|sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SEMA6D, check out the SEMA6D Infographic

SEMA6D infographic

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In this infographic, you will see the following information for SEMA6D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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