SF3A1 (NM_005877) Human Over-expression Lysate

Lysate of SF3A1 over-expression cell lines, WB control

Transient overexpression lysate of splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1

Product Info Summary

SKU: LS010291
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1

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Product Name

SF3A1 (NM_005877) Human Over-expression Lysate

See all SF3A1 products

SKU/Catalog Number

LS010291

Size

100 µg

Description

Transient overexpression lysate of splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1

Storage & Handling

The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.

Cite This Product

SF3A1 (NM_005877) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010291)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SF3A1 (Source: Uniprot.org, NCBI)

Gene Name

SF3A1

Full Name

Splicing factor 3A subunit 1

Weight

88.886kDa

Alternative Names

pre-mRNA processing 21; pre-mRNA splicing factor SF3a (120 kDa subunit); Prp21; PRPF21; SAP 114; SAP114splicing factor 3a, subunit 1, 120kD; SF3a120; spliceosome associated protein 114; Spliceosome-associated protein 114; splicing factor 3 subunit 1; splicing factor 3A subunit 1; splicing factor 3a, subunit 1, 120kDa SF3A1 PRP21, PRPF21, SAP11420, SF3A1 splicing factor 3a subunit 1 splicing factor 3A subunit 1|SAP 114|pre-mRNA processing 21|pre-mRNA splicing factor SF3a (120 kDa subunit)|spliceosome-associated protein 114|splicing factor 3 subunit 1|splicing factor 3a, subunit 1, 120kD|splicing factor 3a, subunit 1, 120kDa

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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