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Product Info Summary
|Description:||Transient overexpression lysate of SHANK-associated RH domain interactor (SHARPIN)|
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SHARPIN (NM_030974) Human Over-expression Lysate
Transient overexpression lysate of SHANK-associated RH domain interactor (SHARPIN)
Storage & Handling
Cite This Product
SHARPIN (NM_030974) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081858)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SHARPIN Human Over-expression Lysates NM_030974.
Western blot validation of overexpression lysate (LS081858) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SHARPIN (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SHARPIN (Source: Uniprot.org, NCBI)
DKFZp434N1923; hSIPL1; SHANK-associated RH domain interactor; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; SHARPIN; SIPL1; SIPL1DKFZP434N1923 SHARPIN SIPL1 SHANK associated RH domain interactor sharpin|hSIPL1|shank-associated RH domain-interacting protein|shank-interacting protein-like 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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