SNRPB (NM_198216) Human Over-expression Lysate

Lysate of SNRPB over-expression cell lines, WB control

Transient overexpression lysate of small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1

Product Info Summary

SKU: LS006628
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1

Product Name

SNRPB (NM_198216) Human Over-expression Lysate

See all SNRPB products

SKU/Catalog Number

LS006628

Size

100 µg

Description

Transient overexpression lysate of small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB), transcript variant 1

Storage & Handling

Cite This Product

SNRPB (NM_198216) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006628)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SNRPB (Source: Uniprot.org, NCBI)

Gene Name

SNRPB

Full Name

Small nuclear ribonucleoprotein-associated proteins B and B'

Weight

24.61kDa

Superfamily

snRNP SmB/SmN family

Alternative Names

CODB polypeptide of Sm protein; Sm protein B/B'; small nuclear ribonucleoprotein polypeptides B and B'; small nuclear ribonucleoprotein polypeptides B and B1; small nuclear ribonucleoprotein-associated proteins B and B'; SmB/B'; Sm-B/B'sm-B/Sm-B'; SmB/SmB'; snRNP-Bsmall nuclear ribonucleoprotein polypeptide B; SNRPB1 SNRPB CCMS, COD1, Sm-B/B, SmB/B, SmB/SmB, snRNP-B, SNRPB small nuclear ribonucleoprotein polypeptides B and B1 small nuclear ribonucleoprotein-associated proteins B and B|B polypeptide of Sm protein|Sm protein B/B|sm-B/Sm-B|small nuclear ribonucleoprotein polypeptide B|small nuclear ribonucleoprotein polypeptides B and B

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SNRPB, check out the SNRPB Infographic

SNRPB infographic

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In this infographic, you will see the following information for SNRPB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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