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Product Info Summary
|Description:||Transient overexpression lysate of Sjogren syndrome antigen B (autoantigen La) (SSB)|
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SSB (NM_003142) Human Over-expression Lysate
Transient overexpression lysate of Sjogren syndrome antigen B (autoantigen La) (SSB)
Storage & Handling
Cite This Product
SSB (NM_003142) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006741)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SSB Human Over-expression Lysates NM_003142.
Western blot validation of overexpression lysate (LS006741) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SSB (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SSB (Source: Uniprot.org, NCBI)
Lupus La protein
La autoantigen; La ribonucleoprotein; lupus La protein; member 3; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B (autoantigen La); SS-B; SS-B/La protein SSB LARP3, La, La/SSB small RNA binding exonuclease protection factor La lupus La protein|La ribonucleoprotein domain family, member 3|SS-B|SS-B/La protein|Sjogren syndrome antigen B|autoantigen La|la autoantigen|lupus La antigen|sjoegren syndrome type B antigen*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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In this infographic, you will see the following information for SSB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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