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Product Info Summary
|Description:||Transient overexpression lysate of signal transducer and activator of transcription 4 (STAT4)|
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STAT4 (NM_003151) Human Over-expression Lysate
Transient overexpression lysate of signal transducer and activator of transcription 4 (STAT4)
Storage & Handling
Cite This Product
STAT4 (NM_003151) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006775)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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STAT4 Human Over-expression Lysates NM_003151.
Western blot validation of overexpression lysate (LS006775) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with STAT4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for STAT4 (Source: Uniprot.org, NCBI)
Signal transducer and activator of transcription 4
transcription factor STAT family
signal transducer and activator of transcription 4; SLEB11; STAT4 STAT4 SLEB11 signal transducer and activator of transcription 4 signal transducer and activator of transcription 4|signal transducer and activator of transcription 4 variant 3*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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