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Product Info Summary
|Description:||Transient overexpression lysate of supervillin (SVIL), transcript variant 1|
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Supervillin (SVIL) (NM_003174) Human Over-expression Lysate
Transient overexpression lysate of supervillin (SVIL), transcript variant 1
Storage & Handling
Cite This Product
Supervillin (SVIL) (NM_003174) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006840)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Supervillin (SVIL) Human Over-expression Lysates NM_003174.
Western blot validation of overexpression lysate (LS006840) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SVIL (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SVIL (Source: Uniprot.org, NCBI)
Archvillin; DKFZp686A17191; membrane-associated F-actin binding protein p205; p205/p250; Supervillin; SVIL SVIL MFM10 supervillin supervillin|archvillin|membrane-associated F-actin binding protein p205|p205/p250*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SVIL, check out the SVIL Infographic
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In this infographic, you will see the following information for SVIL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS006840
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