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Product Info Summary
|Description:||Transient overexpression lysate of syndecan 4 (SDC4)|
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Syndecan 4 (SDC4) (NM_002999) Human Over-expression Lysate
Transient overexpression lysate of syndecan 4 (SDC4)
Storage & Handling
Cite This Product
Syndecan 4 (SDC4) (NM_002999) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006385)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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Syndecan 4 (SDC4) Human Over-expression Lysates NM_002999.
Western blot validation of overexpression lysate (LS006385) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SDC4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SDC4 (Source: Uniprot.org, NCBI)
syndecan proteoglycan family
Amphiglycan; MGC22217; Ryudocan core protein; Ryudocan; SDC4; SYND4ryudocan amphiglycan; syndecan 4 (amphiglycan, ryudocan); syndecan 4; syndecan proteoglycan 4; Syndecan4; Syndecan-4 SDC4 SYND4 syndecan 4 syndecan-4|amphiglycan|ryudocan amphiglycan|ryudocan core protein|syndecan 4 (amphiglycan, ryudocan)|syndecan proteoglycan 4*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SDC4, check out the SDC4 Infographic
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In this infographic, you will see the following information for SDC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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