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Product Info Summary
|Description:||Transient overexpression lysate of transformer 2 beta homolog (Drosophila) (TRA2B)|
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TRA2B (NM_004593) Human Over-expression Lysate
Transient overexpression lysate of transformer 2 beta homolog (Drosophila) (TRA2B)
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Cite This Product
TRA2B (NM_004593) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006434)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TRA2B Human Over-expression Lysates NM_004593.
Western blot validation of overexpression lysate (LS006434) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TRA2B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TRA2B (Source: Uniprot.org, NCBI)
Transformer-2 protein homolog beta
splicing factor SR family
DKFZp686F18120; Htra2-beta; SFRS10; splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila); Splicing factor, arginine/serine-rich 10; SRFS10; TRA-2 beta; TRA2-BETA; TRAN2B; transformer 2 beta homolog (Drosophila); transformer 2 homolog; Transformer-2 protein homolog B; transformer-2 protein homolog beta; transformer-2-beta TRA2B Htra2-beta, PPP1R156, SFRS10, SRFS10, TRA2-BETA, TRAN2B transformer 2 beta homolog transformer-2 protein homolog beta|TRA-2 beta|protein phosphatase 1, regulatory subunit 156|splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila)|transformer-2 protein homolog B*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006434
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