Product Info Summary
SKU: | LS009618 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of TNF receptor-associated factor 4 (TRAF4) |
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Product info
Product Name
TRAF4 (NM_004295) Human Over-expression Lysate
SKU/Catalog Number
LS009618
Size
100 µg
Description
Transient overexpression lysate of TNF receptor-associated factor 4 (TRAF4)
Storage & Handling
Cite This Product
TRAF4 (NM_004295) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009618)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
TRAF4 Human Over-expression Lysates NM_004295.
Western blot validation of overexpression lysate (LS009618) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TRAF4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TRAF4 (Source: Uniprot.org, NCBI)
Gene Name
TRAF4
Full Name
TNF receptor-associated factor 4
Weight
53.543kDa
Superfamily
TNF receptor-associated factor family
Alternative Names
CART1; CART1RING finger protein 83; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; MLN62; MLN62cysteine-rich domain associated with ring and TRAF domain; RNF83malignant 62; TNF receptor-associated factor 4; TRAF4; TRAF-4; tumor necrosis receptor-associated factor 4A TRAF4 CART1, MLN62, RNF83 TNF receptor associated factor 4 TNF receptor-associated factor 4|MLN 62|RING finger protein 83|TRAF4 variant 6|cysteine-rich domain associated with RING and Traf domains protein 1|cysteine-rich domain associated with ring and TRAF domain|malignant 62|metastatic lymph node gene 62 protein|tumor necrosis receptor-associated factor 4A
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TRAF4, check out the TRAF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRAF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TRAF4 (NM_004295) Human Over-expression Lysate (LS009618)
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No publications found for LS009618
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