URP2 (FERMT3) (NM_031471) Human Over-expression Lysate

Lysate of FERMT3/URP2 over-expression cell lines, WB control

Transient overexpression lysate of fermitin family homolog 3 (Drosophila) (FERMT3), transcript variant URP2SF

Product Info Summary

SKU: LS083706
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of fermitin family homolog 3 (Drosophila) (FERMT3), transcript variant URP2SF

Product Name

URP2 (FERMT3) (NM_031471) Human Over-expression Lysate

See all FERMT3/URP2 products

SKU/Catalog Number

LS083706

Size

100 µg

Description

Transient overexpression lysate of fermitin family homolog 3 (Drosophila) (FERMT3), transcript variant URP2SF

Storage & Handling

Cite This Product

URP2 (FERMT3) (NM_031471) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083706)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for FERMT3 (Source: Uniprot.org, NCBI)

Gene Name

FERMT3

Full Name

Fermitin family homolog 3

Weight

75.953kDa

Superfamily

kindlin family

Alternative Names

fermitin family member 3; FERMT3; KIND3; KIND3fermitin family homolog 3 (Drosophila); Kindlin 3; Kindlin-3; MGC10966; MIG-2; MIG2B; MIG2BURP2SF; MIG2-like protein; UNC-112 related protein 2; UNC112C; Unc-112-related protein 2; URP2; URP2fermitin family homolog 3; URP2SF FERMT3 KIND3, MIG-2, MIG2B, UNC112C, URP2, URP2SF FERM domain containing kindlin 3 fermitin family homolog 3|MIG2-like protein|UNC-112 related protein 2|fermitin family member 3|kindlin 3|unc-112-related protein 2

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on FERMT3, check out the FERMT3 Infographic

FERMT3 infographic

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In this infographic, you will see the following information for FERMT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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