AIRE Colorimetric Cell-Based ELISA Kit

Autoimmune Regulator/AIRE ELISA kit, cell-based

The AIRE Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AIRE protein expression profile in cells. The kit can be used for measuring the relative amounts of AIRE in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AIRE.

Product Info Summary

SKU: EKC1020
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA
Sample Types: Cell lines

Product Name

AIRE Colorimetric Cell-Based ELISA Kit

See all Autoimmune Regulator/AIRE products

SKU/Catalog Number

EKC1020

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The AIRE Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AIRE protein expression profile in cells. The kit can be used for measuring the relative amounts of AIRE in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AIRE.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

AIRE Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1020)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1020 is reactive to AIRE in Human, Mouse samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1020 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Autoimmune Regulator/AIRE Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Gene/Protein Information For AIRE (Source: Uniprot.Org, NCBI)

Gene Name

AIRE

Full Name

Autoimmune regulator

Weight

57.727kDa

Alternative Names

AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); Autoimmune Regulator; PGA1 AIRE AIRE1, APECED, APS1, APSI, PGA1 autoimmune regulator autoimmune regulator|autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AIRE, check out the AIRE Infographic

AIRE infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AIRE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EKC1020

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used AIRE Colorimetric Cell-Based ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review AIRE Colorimetric Cell-Based ELISA Kit

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for AIRE Colorimetric Cell-Based ELISA Kit

$480
Backordered.

Lead time for this item is typically 5-7 days

Get A Quote
Eddy test
In stock
Order Product
EKC1020
$480.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.