Product Info Summary
SKU: | PB9980 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-AIRE Antibody Picoband™
View all Autoimmune Regulator/AIRE Antibodies
SKU/Catalog Number
PB9980
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AIRE Antibody Picoband™ catalog # PB9980. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AIRE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9980)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AIRE recombinant protein (Position: M1-K120). Human AIRE shares 87.2% amino acid (aa) sequence identity with mouse AIRE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9980 is reactive to AIRE in Human, Rat
Applications
PB9980 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
57.727kDa
Background of Autoimmune Regulator/AIRE
The autoimmune regulator (AIRE) is a protein that in humans is encoded by the AIRE gene. This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry
(Paraffin-embedded Section), 2-5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AIRE using anti-AIRE antibody (PB9980).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIRE antigen affinity purified polyclonal antibody (Catalog # PB9980) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIRE at approximately 70 kDa. The expected band size for AIRE is at 58 kDa.
Click image to see more details
Figure 2. IHC analysis of AIRE using anti-AIRE antibody (PB9980).
AIRE was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AIRE Antibody (PB9980) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For AIRE (Source: Uniprot.org, NCBI)
Gene Name
AIRE
Full Name
Autoimmune regulator
Weight
57.727kDa
Alternative Names
AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); Autoimmune Regulator; PGA1 AIRE AIRE1, APECED, APS1, APSI, PGA1 autoimmune regulator autoimmune regulator|autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AIRE, check out the AIRE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AIRE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AIRE Antibody Picoband™ (PB9980)
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No publications found for PB9980
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-AIRE Antibody Picoband™
Question
Would anti-AIRE antibody PB9980 work on monkey Flow Cytometry with amniotic fluid?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Our lab technicians have not validated anti-AIRE antibody PB9980 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-AIRE antibody PB9980 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey amniotic fluid in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-19
Question
We are currently using anti-AIRE antibody PB9980 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on primate tissues as well?
L. Dhar
Verified customer
Asked: 2018-11-12
Answer
The anti-AIRE antibody (PB9980) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-12
Question
Will PB9980 anti-AIRE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Evans
Verified customer
Asked: 2017-10-16
Answer
You can see on the product datasheet, PB9980 anti-AIRE antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-16