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Product Info Summary
| SKU: | A01687 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-14-3-3 epsilon YWHAE Antibody
View all 14-3-3 epsilon Antibodies
SKU/Catalog Number
A01687
Size
100μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 epsilon YWHAE Antibody catalog # A01687. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 epsilon YWHAE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01687)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2C5
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human DNA Ligase IV. at AA rangle: 560-640
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01687 is reactive to YWHAE in Human, Mouse, Rat
Applications
A01687 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
29.174kDa
Background of 14-3-3 epsilon
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using 14-3-3 epsilon Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For YWHAE (Source: Uniprot.org, NCBI)
Gene Name
YWHAE
Full Name
14-3-3 protein epsilon
Weight
29.174kDa
Superfamily
14-3-3 family
Alternative Names
1433 epsilon; 14-3-3 epsilon; 14-3-3 protein epsilon; FLJ45465; FLJ53559,14-3-3E; KCIP-1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide; YWHAE YWHAE 14-3-3E, HEL2, KCIP-1, MDCR, MDS tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon 14-3-3 protein epsilon|14-3-3 epsilon|epididymis luminal protein 2|mitochondrial import stimulation factor L subunit|protein kinase C inhibitor protein-1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide|tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YWHAE, check out the YWHAE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YWHAE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-14-3-3 epsilon YWHAE Antibody (A01687)
Hello CJ!
No publications found for A01687
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-14-3-3 epsilon YWHAE Antibody
Question
Is there a BSA free version of anti-14-3-3 epsilon antibody A01687 available?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-14-3-3 epsilon antibody A01687 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-14-3-3 epsilon antibody for WB for rat melanoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat melanoma identification?
P. Evans
Verified customer
Asked: 2019-06-20
Answer
You can see on the product datasheet, A01687 anti-14-3-3 epsilon antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-14-3-3 epsilon antibody A01687. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-30
