Anti-4-1BBL/Tnfsf9 Antibody Picoband™

Product Name

Anti-4-1BBL/Tnfsf9 Antibody Picoband™

View all 4-1BB Ligand/TNFSF9 Antibodies

SKU/Catalog Number

A06032-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-4-1BBL/Tnfsf9 Antibody Picoband™ catalog # A06032-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-4-1BBL/Tnfsf9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06032-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse 4-1BBL/Tnfsf9 recombinant protein (Position: M1-E309).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06032-2 is reactive to TNFSF9 in Mouse, Rat

Applications

A06032-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

26.625kDa

Background of 4-1BB Ligand/TNFSF9

Tumor necrosis factor ligand superfamily member 9 also known as 4-1BB ligand or 4-1BBL or CD137L is a protein that in humans is encoded by the TNFSF9 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of 4-1BBL/Tnfsf9 using anti-4-1BBL/Tnfsf9 antibody (A06032-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-4-1BBL/Tnfsf9 antigen affinity purified polyclonal antibody (Catalog # A06032-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 4-1BBL/Tnfsf9 at approximately 27 kDa. The expected band size for 4-1BBL/Tnfsf9 is at 27 kDa.

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Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-4-1BBL/Tnfsf9 antibody (A06032-2).
Overlay histogram showing ANA-1 cells stained with A06032-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-4-1BBL/Tnfsf9 Antibody (A06032-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For TNFSF9 (Source: Uniprot.org, NCBI)

Alternative Names

41BB Ligand; 4-1BB Ligand; 4-1BBL; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand superfamily member 9 TNFSF9 4-1BB-L, CD137L, TNLG5A TNF superfamily member 9 tumor necrosis factor ligand superfamily member 9|4-1BB ligand|4-1BBL|homolog of mouse 4-1BB-L|receptor 4-1BB ligand|tumor necrosis factor (ligand) superfamily, member 9|tumor necrosis factor ligand 5A|tumor necrosis factor superfamily member 9

For more info on TNFSF9, check out the TNFSF9 Infographic

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In this infographic, you will see the following information for TNFSF9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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