Anti-5HT2B Receptor/HTR2B Antibody Picoband™

Product Name

Anti-5HT2B Receptor/HTR2B Antibody Picoband™

View all 5-HT2B Antibodies

SKU/Catalog Number

PB9600

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-5HT2B Receptor/HTR2B Antibody Picoband™ catalog # PB9600. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-5HT2B Receptor/HTR2B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9600)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2B Receptor, different from the related mouse sequence by six amino acids, and from the related rat sequence by nine amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9600 is reactive to HTR2B in Human, Rat

Applications

PB9600 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

54.298kDa

Background of 5-HT2B

5HT2B Receptor is known as HTR2B. This gene encodes one of the several different receptors for 5-hydroxytryptamine (serotonin) that belongs to the G-protein coupled receptor 1 family. Serotonin is a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin, including regulation of cardiovascular functions and impulsive behavior. Population and family-based analyses of a minor allele (glutamine-to-stop substitution, designated Q20*) which blocks expression of this protein, and knockout studies in mice, suggest a role for this gene in impulsivity. However, other factors, such as elevated testosterone levels, may also be involved.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of 5HT2B Receptor using anti-5HT2B Receptor antibody (PB9600).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: U87 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2B Receptor antigen affinity purified polyclonal antibody (Catalog # PB9600) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2B Receptor at approximately 54 kDa. The expected band size for 5HT2B Receptor is at 54 kDa.

Gene/Protein Information For HTR2B (Source: Uniprot.org, NCBI)

Alternative Names

5-HT(2B); 5HT2B; 5-HT2B; 5-HT-2B; 5-HT2B5-HT 2B receptor; 5-hydroxytryptamine (serotonin) receptor 2B; 5-hydroxytryptamine 2B receptor; 5-hydroxytryptamine receptor 2B; HTR2B; Serotonin receptor 2B HTR2B 5-HT(2B), 5-HT-2B, 5-HT2B 5-hydroxytryptamine receptor 2B 5-hydroxytryptamine receptor 2B|5-HT 2B receptor|5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled|5-hydroxytryptamine 2B receptor|5-hydroxytryptamine receptor 2B variant b|serotonin receptor 2B

For more info on HTR2B, check out the HTR2B Infographic

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In this infographic, you will see the following information for HTR2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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