Product Info Summary
SKU: | A03163 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-ABCA7 Antibody
SKU/Catalog Number
A03163
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ABCA7 Antibody (Catalog # A03163). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
ABCA7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ABCA7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03163)
Host
Rabbit
Contents
ABCA7 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ABCA7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ABCA7. The immunogen is located within amino acids 130 - 180 of ABCA7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ABCA7 antibody is predicted to not cross-react with other ABC1 subfamily proteins.
Reactive Species
A03163 is reactive to ABCA7 in Human
Applications
A03163 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
234.35kDa
Background of ABCA7
ATP-binding cassette (ABC) transporters are an evolutionarily conserved family that use ATP hydrolysis to catalyze the transport of various molecules across cell membranes. They are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). ABCA7, a member of the ABC1 subfamily, is a full-size ABC transporter consisting of two sets of the multiple membrane-spanning domains plus the Walker motifs for the ATP interaction. ABCA7 shows the highest homology to ABCA1, an essential molecule for cholesterol homeostasis. The high expression levels of ABCA7 in peripheral leukocytes, thymus, spleen and bone marrow suggests a role in the immune system lipid homeostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ABCA7 antibody can be used for detection of ABCA7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 μg/mL.
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Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 μg/mL.
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Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 μg/mL.
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Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5μg/ml.
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Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 μg/ml.
Red: ABCA7 Antibody (A03163)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For ABCA7 (Source: Uniprot.org, NCBI)
Gene Name
ABCA7
Full Name
Phospholipid-transporting ATPase ABCA7
Weight
234.35kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABCA-SSN; ABCXATP-binding cassette sub-family A member 7; ATP-binding cassette, sub-family A (ABC1), member 7; Autoantigen SS-N; EC 2.2.1.1; EC 3.6.3; EC 3.6.3.41; FLJ40025; Macrophage ABC transporter ABCA7 ABCA-SSN, ABCX, AD9 ATP binding cassette subfamily A member 7 phospholipid-transporting ATPase ABCA7|ATP-binding cassette sub-family A member 7|ATP-binding cassette, sub-family A (ABC1), member 7|autoantigen SS-N|macrophage ABC transporter
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCA7, check out the ABCA7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCA7 Antibody (A03163)
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No publications found for A03163
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