Anti-ACCN1/ASIC2 Antibody Picoband™

Product Name

Anti-ACCN1/ASIC2 Antibody Picoband™

View all ACCN1 Antibodies

SKU/Catalog Number

PB10046

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ACCN1/ASIC2 Antibody Picoband™ catalog # PB10046. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ACCN1/ASIC2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10046)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ACCN1, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10046 is reactive to ASIC2 in Human, Rat

Applications

PB10046 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

57.709kDa

Background of ACCN1

Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2, is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ACCN1 using anti-ACCN1 antibody (PB10046).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACCN1 antigen affinity purified polyclonal antibody (Catalog # PB10046) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACCN1 at approximately 65 kDa. The expected band size for ACCN1 is at 58 kDa.

Gene/Protein Information For ASIC2 (Source: Uniprot.org, NCBI)

Alternative Names

Acid-sensing ion channel 2; Amiloride-sensitive brain sodium channel; amiloride-sensitive cation channel 1, neuronal; ASIC2a; ASIC2Mammalian degenerin homolog; BNAC1; BNaC1ACCN; BNC1Amiloride-sensitive cation channel neuronal 1; degenerin; hBNaC1; MDEGBrain sodium channel 1; neuronal amiloride-sensitive cation channel 1 ASIC2 ACCN, ACCN1a, BNC1, BNaC1, MDEG, hBNaC1, ASIC2 acid sensing ion channel subunit 2 acid-sensing ion channel 2|acid sensing (proton gated) ion channel 2|amiloride-sensitive cation channel 1, neuronal|brain sodium channel 1|mammalian degenerin homolog|neuronal amiloride-sensitive cation channel 1

For more info on ASIC2, check out the ASIC2 Infographic

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In this infographic, you will see the following information for ASIC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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