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Product Info Summary
| SKU: | M12718 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Acetyl-Histone H2B (Lys20) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM235
SKU/Catalog Number
M12718
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Acetyl-Histone H2B (Lys20) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM235 (Catalog # M12718). Tested in WB, ELISA, Luminex, ICC applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acetyl-Histone H2B (Lys20) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM235 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12718)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM235
Isotype
Rabbit IgG
Immunogen
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20).
Cross-reactivity
This antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross-reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Reactive Species
M12718 is reactive to HIST1H2BB in Human, Vertebrates
Calculated molecular weight
14.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12718 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 ug/mL – 2 ug/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 0.5 ug/mL
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using RM235 at 0.5 ug/mL.
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Specificity Test result
RM235 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
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ICC result
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody (Clone RM235) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Specific Publications For Anti-Acetyl-Histone H2B (Lys20) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM235 (M12718)
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