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Product Info Summary
| SKU: | A03519-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-ACHE Antibody (N-term)
View all Acetylcholinesterase/ACHE Antibodies
SKU/Catalog Number
A03519-1
Size
80 µl
Description
Boster Bio Anti-ACHE Antibody (N-term) (Catalog # A03519-1). Tested in WB, IF, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ACHE Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03519-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ACHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the N-terminal region of human ACHE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03519-1 is reactive to ACHE in Human
Applications
A03519-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
67.796kDa
Background of Acetylcholinesterase/ACHE
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of ACHE Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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ACHE Antibody (N-term) western blot analysis in Jurkat,Raji,Y79 cell line lysates (35ug/lane).This demonstrates the ACHE antibody detected the ACHE protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ACHE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ACHE Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For ACHE (Source: Uniprot.org, NCBI)
Gene Name
ACHE
Full Name
Acetylcholinesterase
Weight
67.796kDa
Superfamily
type-B carboxylesterase/lipase family
Alternative Names
acetylcholinesterase (Yt blood group); Acetylcholinesterase; ACHE; apoptosis-related acetylcholinesterase; ARACHE; EC 3.1.1; EC 3.1.1.7; N-ACHE; Yt blood group; YT ACHE ACEE, ARACHE, N-ACHE, YT acetylcholinesterase (Cartwright blood group) acetylcholinesterase|Yt blood group|acetylcholinesterase (Yt blood group)|apoptosis-related acetylcholinesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACHE, check out the ACHE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACHE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACHE Antibody (N-term) (A03519-1)
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No publications found for A03519-1
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