Anti-ACSL1 Antibody Picoband™

ACSL1 antibody

Boster Bio Anti-ACSL1 Antibody Picoband™ catalog # PB10025. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10025
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-ACSL1 Antibody Picoband™

View all ACSL1 Antibodies

SKU/Catalog Number

PB10025

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ACSL1 Antibody Picoband™ catalog # PB10025. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ACSL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10025)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10025 is reactive to ACSL1 in Human, Mouse, Rat

Applications

PB10025 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

78 kDa

Calculated molecular weight

77.943kDa

Background of ACSL1

Long-chain-fatty-acid—CoA ligase 1 is an enzyme that in humans is encoded by the ACSL1 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. This specific protein is most commonly found in mitochondria and peroxisomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACSL1 (Source: Uniprot.org, NCBI)

Gene Name

ACSL1

Full Name

Long-chain-fatty-acid--CoA ligase 1

Weight

77.943kDa

Superfamily

ATP-dependent AMP-binding enzyme family

Alternative Names

ACS1LACS 2; Acyl-CoA synthetase 1; acyl-CoA synthetase long-chain family member 1; EC 6.2.1; FACL1EC 6.2.1.3; fatty-acid-Coenzyme A ligase, long-chain 1; LACS 1; LACSlong-chain 2; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 1; Long-chain acyl-CoA synthetase 2; Long-chain fatty acid-CoA ligase 2; long-chain fatty-acid-coenzyme A ligase 1; long-chain-fatty-acid--CoA ligase 1; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; paltimoyl-CoA ligase 1 ACSL1 ACS1, FACL1, FACL2, LACS, LACS1, LACS2 acyl-CoA synthetase long chain family member 1 long-chain-fatty-acid--CoA ligase 1|LACS 1|LACS 2|acyl-CoA synthetase 1|arachidonate--CoA ligase|fatty-acid-Coenzyme A ligase, long-chain 1|fatty-acid-Coenzyme A ligase, long-chain 2|lignoceroyl-CoA synthase|long-chain acyl-CoA synthetase 1|long-chain acyl-CoA synthetase 2|long-chain fatty acid-CoA ligase 2|long-chain fatty-acid-coenzyme A ligase 1|palmitoyl-CoA ligase 1|palmitoyl-CoA ligase 2|paltimoyl-CoA ligase 1|phytanate--CoA ligase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACSL1, check out the ACSL1 Infographic

ACSL1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACSL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-ACSL1 Antibody Picoband™

Question

We are currently using anti-ACSL1 antibody PB10025 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The anti-ACSL1 antibody (PB10025) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-09

Question

See below the WB image, lot number and protocol we used for liver using anti-ACSL1 antibody PB10025. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-04-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-04

Question

I was wanting to use your anti-ACSL1 antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

C. Baker

Verified customer

Asked: 2017-01-31

Answer

You can see on the product datasheet, PB10025 anti-ACSL1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-01-31

Size

Conjugation

Total: $370

SKU:PB10025

In stock, 1 left.

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PB10025
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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