Product Info Summary
SKU: | M05553 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ACTL6A Monoclonal Antibody
SKU/Catalog Number
M05553
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ACTL6A Monoclonal Antibody catalog # M05553. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTL6A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05553)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFCF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ACTL6A Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) . Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05553 is reactive to ACTL6A in Human, Mouse, Rat
Applications
M05553 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
10 kDa
Calculated molecular weight
47.461kDa
Background of BAF53A
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ACTL6A expression in Ramos cell lysate.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For ACTL6A (Source: Uniprot.org, NCBI)
Gene Name
ACTL6A
Full Name
Actin-like protein 6A
Weight
47.461kDa
Superfamily
actin family
Alternative Names
actin-like 6A; actin-like protein 6A; actin-related protein 4,53 kDa BRG1-associated factor A; Actin-related protein Baf53a; Actl6; Arp4; arpNbeta; BAF complex 53 kDa subunit; Baf53a; BAF53AACTL6; BAF53MGC5382; BRG1-associated factor 53A; hArpN beta; INO80 complex subunit K; INO80KARPN-BETA ACTL6A ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K actin like 6A actin-like protein 6A|53 kDa BRG1-associated factor A|BAF complex 53 kDa subunit|BAF53|BRG1-associated factor 53A|INO80 complex subunit K|actin-related protein 4|actin-related protein Baf53a|arpNbeta|hArpN beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTL6A, check out the ACTL6A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTL6A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACTL6A Monoclonal Antibody (M05553)
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No publications found for M05553
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-ACTL6A Monoclonal Antibody
Question
I was wanting to use your anti-ACTL6A Monoclonal antibody for IP for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
It shows on the product datasheet, M05553 anti-ACTL6A Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
We are currently using anti-ACTL6A Monoclonal antibody M05553 for rat tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-ACTL6A Monoclonal antibody (M05553) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
Does anti-ACTL6A Monoclonal antibody M05553 work for IP with testis?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
According to the expression profile of testis, ACTL6A is highly expressed in testis. So, it is likely that anti-ACTL6A Monoclonal antibody M05553 will work for IP with testis.
Boster Scientific Support
Answered: 2017-11-15