Anti-ADAM19 Antibody

Product Name

Anti-ADAM19 Antibody

View all ADAM19 Antibodies

SKU/Catalog Number

PA2070

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAM19 Antibody catalog # PA2070. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAM19 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2070)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ADAM19, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2070 is reactive to ADAM19 in Human

Applications

PA2070 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

105 kDa

Calculated molecular weight

104.997kDa

Background of ADAM19

ADAM19 (A Disintegrin and Metalloproteinase Domain 19), also known as MLTNB, is a human gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. Hirohata et al. (1998) used radiation hybrids to map ADAM19 to mouse chromosome 11 and to human chromosome 5q32-q33.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ADAM19 using anti-ADAM19 antibody (PA2070).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM19 antigen affinity purified polyclonal antibody (Catalog # PA2070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM19 at approximately 105 kDa. The expected band size for ADAM19 is at 105 kDa.

Gene/Protein Information For ADAM19 (Source: Uniprot.org, NCBI)

Alternative Names

a disintegrin and metalloproteinase domain 19 (meltrin beta); ADAM 19; ADAM metallopeptidase domain 19; ADAM19; disintegrin and metalloproteinase domain-containing protein 19; EC 3.4.24; EC 3.4.24.-; MADDAM; Meltrin beta; meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker; MLTNBmeltrin beta ADAM19 FKSG34, MADDAM, MLTNB ADAM metallopeptidase domain 19 disintegrin and metalloproteinase domain-containing protein 19|a disintegrin and metalloproteinase domain 19 (meltrin beta)|metalloprotease and disintegrin dendritic antigen marker|metalloprotease-disintegrin meltrin beta

For more info on ADAM19, check out the ADAM19 Infographic

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In this infographic, you will see the following information for ADAM19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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