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Product Info Summary
| SKU: | A05106-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Adducin Beta (E497) ADD2 Antibody
SKU/Catalog Number
A05106-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Adducin Beta (E497) ADD2 Antibody catalog # A05106-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adducin Beta (E497) ADD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05106-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 470-520 of Human Adducin β.
Reactive Species
A05106-1 is reactive to ADD2 in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
80.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05106-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of Adducin β (E497) pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:The Brain tissue lysate of Mouse(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Specific Publications For Anti-Adducin Beta (E497) ADD2 Antibody (A05106-1)
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