Product Info Summary
SKU: | M04795 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Adenylate Cyclase III Antibody
View all Adenylate Cyclase 3 Antibodies
SKU/Catalog Number
M04795
Size
100ul
Description
Boster Bio Anti-Adenylate Cyclase III Antibody catalog # M04795. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adenylate Cyclase III Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04795)
Host
Rabbit
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Polyclonal
Immunogen
C-terminal peptide of rat ACIII, PAAFPNGSSVTLPHQVVDNP with a Cys added to the N-terminus to allow coupling to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04795 is reactive to ADCY3 in Human, Mouse, Rat
Applications
M04795 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
128.96kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000. IF/ICC or IHC: 1:10,000-20,000.
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence of rat hippocampus stained with M04795 antibody to adenylate cyclase III in red and mouse antibody to MECP2 in green, DNA is revealed with DAPI in blue. This is a stacked confocal image made with a 100X objective lens. The ACIII antibody reveals neuronal cilia while the MECP2 antibody reveals the nuclei of certain neurons to a variable degree.
Click image to see more details
Western blot analysis of HEK293 cell lysates using rabbit pAb to ACIII, M04795, dilution 1:2,000, in green: [1] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human adenylate cyclase III cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human ACIII protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of ACIII. The same blot was simultaneously probed with mouse mAb to GAPDH, dilution 1:5,000, in red, which reveals the single band at ~37kDa in both transfected and non-transfected cells.
Protein Target Info & Infographic
Gene/Protein Information For ADCY3 (Source: Uniprot.org, NCBI)
Gene Name
ADCY3
Full Name
Adenylate cyclase type 3
Weight
128.96kDa
Superfamily
adenylyl cyclase class-4/guanylyl cyclase family
Alternative Names
AC3EC 4.6.1.1; AC-III; adenylate cyclase 3; adenylate cyclase type 3; Adenylate cyclase type III; Adenylyl cyclase 3; adenylyl cyclase, type III; ATP pyrophosphate-lyase 3; EC 4.6.1; KIAA0511; olfactive type ADCY3 AC-III, AC3, BMIQ19 adenylate cyclase 3 adenylate cyclase type 3|ATP pyrophosphate-lyase 3|adenylate cyclase type III|adenylate cyclase, olfactive type|adenylyl cyclase 3|adenylyl cyclase, type III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADCY3, check out the ADCY3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADCY3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adenylate Cyclase III Antibody (M04795)
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No publications found for M04795
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Adenylate Cyclase III Antibody
Question
We are currently using anti-Adenylate Cyclase III antibody M04795 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
The anti-Adenylate Cyclase III antibody (M04795) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-27