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Product Info Summary
| SKU: | A05950-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Anterior gradient protein 3 AGR3 Antibody
SKU/Catalog Number
A05950-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Anterior gradient protein 3 AGR3 Antibody (Catalog# A05950-1). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Anterior gradient protein 3 AGR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05950-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AGR3. Immunogen sequence location: 1-50
Reactive Species
A05950-1 is reactive to AGR3 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
19.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05950-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of COS7 cells, using AGR3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AGR3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Anterior gradient protein 3 AGR3 Antibody (A05950-1)
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Customer Q&As
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7 Customer Q&As for Anti-Anterior gradient protein 3 AGR3 Antibody
Question
Is this A05950-1 anti-AGR3 antibody reactive to the isotypes of AGR3?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The immunogen of A05950-1 anti-AGR3 antibody is A peptide derived from human AGR3. Immunogen sequence location: 1-50. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-29
Question
Here is the WB image, lot number and protocol we used for lung using anti-AGR3 antibody A05950-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-AGR3 antibody A05950-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-AGR3 antibody (A05950-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
Question
I was wanting to use to test anti-AGR3 antibody A05950-1 on mouse lung for research purposes, then I may be interested in using anti-AGR3 antibody A05950-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The products we sell, including anti-AGR3 antibody A05950-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-14
Question
Does anti-AGR3 antibody A05950-1 work for IF with lung?
E. Anderson
Verified customer
Asked: 2016-04-22
Answer
According to the expression profile of lung, AGR3 is highly expressed in lung. So, it is likely that anti-AGR3 antibody A05950-1 will work for IF with lung.
Boster Scientific Support
Answered: 2016-04-22
Question
I was wanting to use your anti-AGR3 antibody for IF for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
K. Yang
Verified customer
Asked: 2013-04-29
Answer
It shows on the product datasheet, A05950-1 anti-AGR3 antibody has been tested for IF, IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-29
Question
Do you have a BSA free version of anti-AGR3 antibody A05950-1 available?
C. Dhar
Verified customer
Asked: 2013-04-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-AGR3 antibody A05950-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-22
