Product Info Summary
SKU: | M05950 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Mouse |
Application: | IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-AGR3 Purified Monoclonal Antibody
SKU/Catalog Number
M05950
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AGR3 Purified Monoclonal Antibody (Catalog# M05950). Tested in WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-AGR3 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05950)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
AGR3.1
Isotype
Mouse IgG1
Immunogen
Purified human AGR3 protein. The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa); a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05950 is reactive to AGR3 in Human
Applications
M05950 is guaranteed for IHC-P, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
19.171kDa
Background of AG-3/AGR3
AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 1 μg/ml; positive tissue: breast cancer.
Immunohistochemistry (frozen sections): 1 μg/ml; positive tissue: breast cancer.
Western blotting: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: 1 μg/ml; positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of AGR3 using Anti-AGR3 Purified Monoclonal Antibody (M05950).
Immunohistochemistry staining of AGR3 with mouse monoclonal AGR3.1 in paraffin-embedded sections of breast carcinoma.For more protocol information of IHC
Click image to see more details
Figure 2. Western blotting validation for Anti-AGR3 Purified Monoclonal Antibody M05950
Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibodyProtein Target Info & Infographic
Gene/Protein Information For AGR3 (Source: Uniprot.org, NCBI)
Gene Name
AGR3
Full Name
Anterior gradient protein 3
Weight
19.171kDa
Superfamily
AGR family
Alternative Names
AG3; AG-3; AGR3; anterior gradient homolog 3 (Xenopus laevis); BCMP11; BCMP11AG3; Breast cancer membrane protein 11; hAG-3member 18; PDIA18 AGR3 AG-3, AG3, BCMP11, HAG3, PDIA18, hAG-3 anterior gradient 3, protein disulphide isomerase family member anterior gradient protein 3|anterior gradient homolog 3|anterior gradient protein 3 homolog|breast cancer membrane protein 11|protein disulfide isomerase family A, member 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGR3, check out the AGR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AGR3 Purified Monoclonal Antibody (M05950)
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No publications found for M05950
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