Anti-AHNAK1 Purified Monoclonal Antibody

Product Name

Anti-AHNAK1 Purified Monoclonal Antibody

View all AHNAK Antibodies

SKU/Catalog Number

M05378

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-AHNAK1 Purified Monoclonal Antibody (Catalog# M05378). Tested in WB, IHC-F, ICC, IP application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-AHNAK1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05378)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

EM-09

Isotype

Mouse IgG1

Immunogen

Bacterially expressed fragment of N-terminal domain of human AHNAK1. The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M05378 is reactive to AHNAK in Human, Mouse

Applications

M05378 is guaranteed for IP, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

629.101kDa

Background of AHNAK

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Positive control: HeLa human cervix carcinoma cell line.

Validation Images & Assay Conditions

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Figure 1. Immunocytochemistry staining of AHNAK using Anti-AHNAK1 Purified Monoclonal Antibody (M05378).

Immunocytochemistry staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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Figure 2. Immunocytochemistry staining of AHNAK using Anti-AHNAK1 Purified Monoclonal Antibody (M05378).

Immunocytochemistry staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).

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Figure 3. Immunohistochemistry validation of AHNAK using Anti-AHNAK1 Purified Monoclonal Antibody (M05378).

Immunohistochemistry staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green)

Gene/Protein Information For AHNAK (Source: Uniprot.org, NCBI)

Alternative Names

AHNAK nucleoprotein (desmoyokin); AHNAK nucleoprotein; AHNAK-related; AHNAKRS; desmoyokin; MGC5395; neuroblast differentiation-associated protein AHNAK; PM227 AHNAK AHNAKRS, PM227 AHNAK nucleoprotein neuroblast differentiation-associated protein AHNAK|AHNAK-related|desmoyokin

For more info on AHNAK, check out the AHNAK Infographic

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In this infographic, you will see the following information for AHNAK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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